![]() ![]() You know, you could see yourself in Robert McCall." But I think that’s what people are connecting to. He seems like somebody you sit down and have a tea with or a friend of yours." Fuqua doesn't dismiss Robert's brutality, but even notes people could connect to that in some way as well: "He just happens to be a brutal destroyer when it comes time to do that. Despite anything in Robert's past or present, he resonates with audiences because "he's a common man." Fuqua adds that, generally, Robert could be anybody's friend, noting that "there’s nothing fancy about him or anything. 2015 11:846.During the Q&A with Collider's Steve Weintraub following the screening, Fuqua shared why he thinks people have helped push the franchise into three movies now. A chemical-genetic interaction map of small molecules using high-throughput imaging in cancer cells. ![]() Image-based multivariate profiling of drug responses from single cells. Cell painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. 2017 171:1437–1452.īray M.A., Singh S., Han H., Davis C.T., Borgeson B., Hartland C., Kost-Alimova M., Gustafsdottir S.M., Gibson C.C., Carpenter A.E. A next generation connectivity Map: L1000 platform and the first 1,000,000 profiles. Subramanian A., Narayan R., Corsello S.M., Peck D.D., Natoli T.E., Lu X., Gould J., Davis J.F., Tubelli A.A., Asiedu J.K. ![]() Data-analysis strategies for image-based cell profiling. Review.Ĭaicedo J.C., Cooper S., Heigwer F., Warchal S., Qiu P., Molnar C., Vasilevich A.S., Barry J.D., Bansal H.S., Kraus O. Keenan AB, Jenkins SL, Jagodnik KM, Koplev S, He E, Torre D, Wang Z, Dohlman AB, Silverstein MC, Lachmann A, Kuleshov MV, Ma'ayan A, Stathias V, Terryn R, Cooper D, Forlin M, Koleti A, Vidovic D, Chung C, Schürer SC, Vasiliauskas J, Pilarczyk M, Shamsaei B, Fazel M, Ren Y, Niu W, Clark NA, White S, Mahi N, Zhang L, Kouril M, Reichard JF, Sivaganesan S, Medvedovic M, Meller J, Koch RJ, Birtwistle MR, Iyengar R, Sobie EA, Azeloglu EU, Kaye J, Osterloh J, Haston K, Kalra J, Finkbiener S, Li J, Milani P, Adam M, Escalante-Chong R, Sachs K, Lenail A, Ramamoorthy D, Fraenkel E, Daigle G, Hussain U, Coye A, Rothstein J, Sareen D, Ornelas L, Banuelos M, Mandefro B, Ho R, Svendsen CN, Lim RG, Stocksdale J, Casale MS, Thompson TG, Wu J, Thompson LM, Dardov V, Venkatraman V, Matlock A, Van Eyk JE, Jaffe JD, Papanastasiou M, Subramanian A, Golub TR, Erickson SD, Fallahi-Sichani M, Hafner M, Gray NS, Lin JR, Mills CE, Muhlich JL, Niepel M, Shamu CE, Williams EH, Wrobel D, Sorger PK, Heiser LM, Gray JW, Korkola JE, Mills GB, LaBarge M, Feiler HS, Dane MA, Bucher E, Nederlof M, Sudar D, Gross S, Kilburn DF, Smith R, Devlin K, Margolis R, Derr L, Lee A, Pillai A. We demonstrate the utility of our method by applying it to cell morphological changes in a human bone osteosarcoma cell line. Additionally, for a new transcriptomic query, our approach can be used to predict associated changes in cellular morphology. To address this, we propose a cell morphology enrichment analysis to assess the association between transcriptomic alterations and changes in cell morphology. Previous methods to investigate cell phenotypes have focused on targeting candidate genes as components of known pathways, RNAi morphological profiling, and cataloging morphological defects however, these methods do not provide an explicit model to link transcriptomic changes with corresponding alterations in morphology. These data provide an opportunity to study the interdependence between transcription and cell morphology. Recently, the Library of Integrated Network-based Cellular Signatures (LINCS) Project has measured gene expression and performed image-based cell profiling on cell lines treated with 9515 unique compounds. Image-based cell profiling or cell morphological profiling has been used to associate changes of cell morphological features with alterations in cellular function and to infer molecular mechanisms of action. Cell morphological phenotypes, including shape, size, intensity, and texture of cellular compartments have been shown to change in response to perturbation with small molecule compounds.
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